Disease. Gummy stem blight. Causes leaf spot, stem canker, black leaf and fruit rot; common name refers to the characteristic gummy exudate. Didymella bryoniae (Auersw.) Rehm [anamorph Phoma cucurbitacearum (Fr.) Sacc.] is the causative agent of gummy stem blight (GSB), a disease affecting. Gummy stem blight of cucurbits produces a variety of symptoms which are referred to as leaf spot, stem canker, vine wilt and black fruit rot. Lesions on leaves.

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Allele-specific PCR for the detection of azoxystrobin resistance in Didymella bryoniae. To our knowledge, this is the first report of gummy stem blight and black fruit rot of watermelon caused by D. On muskmelon, both external and internal rot may occur; black mycelial didjmella of the pathogen may be present in the cavity of some affected fruit.

Gummy stem blight and black rot are caused by the ascomycete fungus Didymella bryoniae Auersw. Although the LAMP assays in this study showed high specificity and sensitivity, and showed the highest detection limit so far in the subfemtogram range Niessen,we still need to be cautious because the presence of calcein may inhibit the LAMP reaction and reduce the sensitivity Wastling et al.

Optimal reaction time of LAMP. Open in a separate window.


Didymella bryoniae

Studies on gummy stem blight disease of cucurbits in Trinidad. The entire procedure is not difficult to perform and requires only an isothermal instrument, such as a water bath diidymella heating block Nagamine et al. First report of gummy stem blight caused by Didymella bryoniae on watermelon and confirmation of the disease on pumpkin in Tanzania.

From Wikipedia, the free encyclopedia. Early symptoms appear as bryoniea or chlorosis of the leaf margins followed by light to dark brown necrotic spots.

Didymella bryoniae

Gummy stem blight of watermelon. Consequently, it is worth emphasizing that the advantages offered by this LAMP assay provide a robust, visual, and easy-to-perform approach for detecting D. Fungal and Culture Conditions Five D.

Drops of gummy, amber plant sap may appear on some cankers under certain, undefined conditions Fig. Bryoniad germinate, and can directly penetrate through the cuticle or intercellular spaces and around the bases of trichomes.

Author information Article notes Copyright and License information Disclaimer. Thus, for the diagnosis method to be used at the agricultural site, a simple, reliable, bryonjae unambiguous visual inspection method is required.

A serious threat to the pumpkin industry. The prospective application of this diagnostic tool for early and accurate detection of the causal agent of GSB in cucurbit crops could also lead to reduced fungicide use, thus benefiting the environment. Along with fungicidesit is important to have proper ventilation and irrigation practices in greenhouse settings [2].


Compendium of Cucurbit Diseases. Using didymellx optimal reaction conditions described above, and based on the presence didymeella a PCR product of the expected size, the D. A rapid detection method for the plant pathogen Sclerotinia sclerotiorum based on loop-mediated isothermal amplification LAMP. The positive reaction becomes yellowish-green, and the negative is still brown; B LAMP product was manifested as a ladder-like pattern on a 2.

Resistant cultivars are currently not available. On the basis of the morphological characteristics, the fungus was identified as Didymella bryoniae Auersw.

Spots on stems often elongate into streaks usually starting at the joints and have an amber exudate of gummy material. Muskmelon genomic DNA was extracted from the leaves. Spots on cotyledons or true leaves also occur in the greenhouse. Diplodina citrullina Chester Grossenb.

Gummy stem blight can occur on seedlings of watermelon, muskmelon, and other cucurbits grown in greenhouses for use as transplants 11, Notes on three new or noteworthy diseases of plants. Development of two alternative Loop-mediated isothermal amplification tools for detecting pathogenic pine wood nematodes.

Differential sensitivity to boscalid in conidia and ascospores of Didymella bryoniae and frequency of boscalid-insensitive isolates in South Carolina.